Learn about detection of novel RNA fusions via new NGS library prep methods with Dr. William Rehrauer on January 24.
RNA Extraction from Formalin Fixed Paraffin Embedded (FFPE) Tissue Enabling Next Generation Detection of Gene Fusions
Among a wide array of genomic alterations, gene fusions can be oncogenic drivers in human cancers usually through production of chimeric proteins with altered functionality. Detection and characterization of gene fusions may provide a more detailed understanding of tumorigenesis, but more importantly can inform direct therapeutic targeting. Next-generation sequencing (NGS) technologies permit identification of fusion genes. Developments in the improvement of RNA extraction from FFPE clinical tissue samples supporting NGS detection of gene fusions will be presented.
Dr. William Rehrauer
Director of the Molecular Diagnostics Clinical Lab
UW Hospital and Clinics
William M. Rehrauer is an Assistant Professor in the Department of Pathology and Laboratory Medicine in the School of Medicine and Public Health at the University of Wisconsin-Madison. He is also Director of the Molecular Diagnostics Clinical Laboratory at the University of Wisconsin Hospital and Clinics and serves as Co-Director of the Translational Research Initiatives in Pathology (TRIP) laboratory. His interests and expertise are in the development of, application of, and education on molecular technologies, in both clinical and translational research laboratories, across such diverse disciplines as infectious disease, genetics, solid tumor oncology and hematopathology. He is an active member in the professional societies of the Association for Molecular Pathology (AMP), the Molecular Pathology Division of the American Association for Clinical Chemistry (AACC) and the American Society of Clinical Pathology (ASCP) and an invited participant in the Roche Molecular Center of Excellence Alliance.