Western Blue Stabilized Substrate for Alkaline Phosphatase is a stable, ready-to-use substrate for Western blots and immunoscreening. It is a mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium (NBT) in a proprietary stabilizing buffer. Western Blue Substrate should be used directly and without dilution. This liquid substrate deposits a permanent dark purple stain on membrane sites bearing alkaline phosphatase. Western Blue Substrate is as sensitive as other reagents based on the BCIP/NBT formulation.