NdeI

by Promega
Available SKUs: R6801 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    CA?TA  TG
    GT   AT?AC

    Notes:

    1. Star activity may be observed with glycerol concentrations >10%.
    2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
    3. Ends generated with NdeI can be directly ligated to ends generated by VspI. Neither recognition site is regenerated in the ligation product.

    Incubation Conditions: Buffer D. 37°C.

    Source: Neisseria denitrificans.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    <10% <10% 25–50% 100% 25–50%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    7 2 0 1 3 1
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other

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