RQ1 RNase-Free DNase

by Promega
Available SKUs: M6101 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO4, 10mM CaCl2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C])

    Applications

    • Preparation of DNA-free RNA.
    • Degradation of DNA from RNA transcription systems.
    • Nick translation of DNA.
    • Studying DNA:protein interactions by DNase I footprinting.

    Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl2, 10mM MgCl2.

    Source: Bovine pancreas.

    QC Tests: Activity, RNase.

    Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1ug of DNA in 10 minutes at 37°C in 50ul of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.

  • Protocols

    Complete Protocol

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    RQ1 RNase-Free DNase Product Information

    PDF (125 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations

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