T4 DNA Ligase

by Promega
Available SKUs: M1801 | M1804 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.

    Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

    Reference

    1. Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY.

    Note: Units listed are Weiss units.

    Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

    Source: Recombinant E. coli strain.

    QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

    Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1ug of ?/HindIII fragments at 16°C in 20 minutes.

  • Protocols

    Complete Protocol

    Download PDF

    T4 DNA Ligase, Blue/White Cloning Qualified Protocol

    PDF (110 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations

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