T4 Polynucleotide Kinase

Description

• Overview
• Protocols
• Specifications
• Resources

• T4 Polynucleotide Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation and cloning. T4 PNK is provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl₂, 50mM DTT.

  Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

  Storage Buffer: 20mM Tris-HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1uM ATP and 50% (v/v) glycerol.

  Source: Recombinant E. coli strain.

  QC Tests: Activity, DNase, RNase, endonuclease, end-labeling, blue/white cloning assay.

  Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5´-OH end of a polynucleotide from [?-³²P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl₂, 5mM DTT, 0.1mM [?-³²P] ATP and 0.5mM 5´-OH polynucleotide end concentration.

• Protocols

  Complete Protocol

  

  T4 Polynucleotide Kinase Technical Bulletin

  PDF (144 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  -30C TO -10C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Citations

  • Nuclear factor-?B and mitogen-activated protein kinases mediate nitric oxide-enhanced transcriptional expression of interferon-k?.
    --
    2003 J. Biol. Chem.