Wizard Genomic DNA Purification Kit

Description

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• Overview
• Protocols
• Specifications
• Resources

• Simple Method to Isolate Genomic DNA from Many Sample Types

  The Wizard® Genomic DNA Purification Kit provides a simple, solution-based method for isolation of DNA from white blood cells, tissue culture cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative bacteria. DNA purified with this system is suitable for a variety of applications, including amplification, digestion with restriction endonucleases and membrane hybridizations (e.g., Southern and dot/slot blots).

  Learn more about our custom options for this product at: www.promega.com/custom/

  Sample Types Processed with the Wizard® Genomic Kit

  Blood

  Sample Type	Promega	External	Comments
  Blood and blood cells
  Human whole blood	Yes	Yes	Protocols are provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050 for samples of up to 10ml blood or in 96-well plates ("Isolating Genomic DNA from Whole Blood" sections). The protocol works with the following anticoagulants: EDTA, heparin and citrate. Reference: Rizzo, W.B., Carney, G. and Lin, Z. (1999) Am. J. Hum. Genet. 65, 1547–60. PubMed ID #10577908
  Mouse whole blood	Yes		A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050.
  Human peripheral blood mononuclear cells		Yes	Tan, A. et al. (2006) Clin. Chem. 52, 2250–7. PubMed ID #17040960
  Bovine whole blood		Yes	Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69. PubMed ID #12711348
  Horse whole blood		Yes	Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69. PubMed ID #12711348
  Sheep whole blood		Yes	Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69. PubMed ID #12711348
  Antelope whole blood		Yes	Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69. PubMed ID #12711348
  Dog whole blood		Yes	Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69. PubMed ID #12711348
  Cat whole blood		Yes	Walker, J.A. et al.(2003) Anal. Biochem. 316, 259–69. PubMed ID #12711348
  Rabbit whole blood		Yes	Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69. PubMed ID #12711348

  Mammalian Tissues

  Sample Type	Promega	External	Comments
  Mammalian tissues
  Mouse liver	Yes	Yes	Tissue needs to be homogenized prior to isolation. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section. Reference: Barazzoni, R. et al. (2005) Endocrinology 146, 2098–106. PubMed ID #15618355.
  Mouse tail	Yes	Yes	Tail needs to be treated with proteinase K before purification. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section. Reference: Nielsen, J.V. et al. (2007) Development 134, 1133–40. PubMed ID #17301088.
  Mouse brain		Yes	Sinz, E.H. et al. (1999) J. Clin. Invest. 104, 647–56. PubMed ID #10487779.
  Mouse Lymphoma		Yes	Yu, Z. et al. (2006) Cancer Res. 66, 755–62. PubMed ID #16424006.
  Rat skeletal muscle, liver and heart		Yes	Barazzoni, R. et al. (2000) J. Biol. Chem. 275, 3343–7. PubMed ID #10652323.
  Human bone marrow aspirate		Yes	Yu, Z. et al. (2006) Cancer Res. 66, 755–62. PubMed ID #16424006.
  Human liver and testicle		Yes	Maduro, M.R. et al. (2003) Mol. Hum. Reprod. 9, 61–8. PubMed ID #12569174.
  Human colon		Yes	Tost, J. et al. (2003) Nucleic Acids Res. 31, e50. PubMed ID #12711695.

  Plant Tissues

  Sample Type	Promega	External	Comments
  Plant tissues
  Tomato leaf	Yes		Leaf needs to be frozen in liquid nitrogen and ground with a mortar and pestle prior to adding lysis buffer. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Plant Tissue" section.
  Arabidopsis		Yes	Vorwerk, S. (2001) Wizard® Genomic DNA Purification Kit and the isolation of plant genomic DNA. eNotes
  Maize		Yes	Vorwerk, S. (2001) Wizard® Genomic DNA Purification Kit and the isolation of plant genomic DNA. eNotes
  Lemna minor (aquatic plant)		Yes	Stout, L.M. and Nüsslein, K. (2005) Appl. Environ. Microbiol. 71, 2484–92. PubMed ID #15870338.
  Medicago truncatula		Yes	Total DNA was isolated from roots, which may include various mycorrhizal fungi. Kosuta, S. et al. (2003) Plant Physiol. 131, 952–62. PubMed ID #12644648.
  Tetrahymena thermophila		Yes	Followed plant DNA protocol ("Isolating Genomic DNA from Plant Tissue"), but skipped freezing in liquid nitrogen and grinding with a mortar and pestle. Jacobs, M.E. et al. (2006) Eukaryot. Cell 5, 1990–2000. PubMed ID #17012537.

  Cultured Cells

  Sample Type	Promega	External	Comments
  Cultured cells (Established cell lines are not listed.)	Yes	Yes	A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section. Reference: Zhang, S. et al. (2006) Cancer Res. 66, 8847–57. PubMed ID #16951202.
  Drosophila D.Mel-2 embryonic cells		Yes	Roberti, M. et al. (2006) Nucleic Acids Res. 34, 2109–16. PubMed ID #16648357.
  Mouse embryonic stem cells		Yes	Oka, M. et al. (2006) J. Biol. Chem. 281, 9901–8. PubMed ID #16439359.
  Monkey primary fibroblasts, virus-infected		Yes	Cells were lysed using 300 µl of Nuclei Lysis Solution with proteinase K and incubating overnight at 37°C. Mansfield, K.G. et al. (1999) J. Virol. 73, 10320–8. PubMed ID #10559350.
  Vero cells infected with HSV-1		Yes	Both viral and genomic DNA were isolated. Bower, J.R. et al. (1999) J. Virol. 73, 3843–53. PubMed ID #10196279.
  CD45RA+ T cells from cord blood		Yes	Samples of 2–4 × 106 cells were pelleted, washed with PBS, snap frozen in liquid nitrogen and frozen at –70°C prior to using the Wizard® Genomic DNA Purification Kit. Soares et al. (1998) J. Immunol. 161, 5909–17. PubMed ID #9834071.

  Bacteria – Gram Negative

  Sample Type	Promega	External	Comments
  Bacteria – Gram Negative
  Enterobacter cloacae	Yes		A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
  Escherichia coli	Yes	Yes	A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section. Reference: Zheng, W. et al. (2001) EMBO J. 20, 1164–72. PubMed ID #11230139.
  Nitrobacter winogradskyi		Yes	Starkenburg, S.R. et al. (2006) Appl. Environ. Microbiol. 72, 2050–63. PubMed ID #16517654.
  Yersinia pestis		Yes	Flashner, Y. et al. (2004) Infect. Immun. 72, 908–15. PubMed ID #14742535.
  Burkholderia species		Yes	Moore, R.A. et al. (2004) Infect. Immun. 72, 4172–87. PubMed ID #15213162.
  Salmonella enterica		Yes	Nair, S. et al. (2004) J. Bacteriol. 186, 3214–23. PubMed ID #15126484.
  Field bacteria samples, non-Agrobacterium		Yes	Weller, S.A. et al. (2004) Appl. Environ. Microbiol. 70, 2779–85. PubMed ID #15128532.
  Haemophilus influenzae		Yes	Ottemann, K.M. and Lowenthal, A.C. (2002) Infect. Immun. 70, 1984–90. PubMed ID #11895962.
  Bordetella bronchiseptica		Yes	Brockmeier, S.L. et al. (2002) Infect. Immun. 70, 481–90. PubMed ID #11796573.
  Leptospira kirschneri		Yes	Matsunaga, J. et al. (2002) Infect. Immun. 70, 323–34. PubMed ID #11748198.
  Klebsiella pneumoniae		Yes	Yigit, H. et al. (2001) Antimicrob. Agents Chemother. 45, 1151–61. PubMed ID #11257029.
  Marine Geobacteraceae		Yes	Bacteria colonizing energy-harvesting anodes were scraped off, organically extracted and purified using the Wizard® Genomic DNA Purification Kit. Bond, D.R. et al. (2002) Science 295, 483–5. PubMed ID #11799240.
  Serratia marcescens		Yes	Peccia, J. and Hernandez, M. (2001) Appl. Environ. Microbiol. 67, 4225–32. PubMed ID #11526027.
  Borrelia burgdorferi		Yes	Chaconas, G. et al. (2001) EMBO J. 20, 3229–37. PubMed ID #11406599.
  Campylobacter jejuni		Yes	Dorrell, N. et al.(2001) Genome Res. 11, 1706–15. PubMed ID #11591647.
  Desulfovibrio desulfuricans		Yes	Rapp-Giles, B.J. et al. (2000) Appl. Environ. Microbiol. 66, 671–7. PubMed ID #10653734.
  Vibrio cholerae		Yes	Stine, O.C. et al. (2000) Infect. Immun. 68, 7180–5. PubMed ID #11083852.
  Vibrio harveyi		Yes	Zhou, W. et al. (1999) Biochemistry 38, 16246–52. PubMed ID #10587447.
  Paracoccus denitrificans		Yes	Shearer, N. et al. (1999) J. Bacteriol. 181, 6907–13. PubMed ID #10559155.
  Campylobacter species		Yes	Before genomic DNA isolation, the bacteria were washed in TE buffer and resuspended in 1ml of TE buffer at ~1 × 109 cells/ml. Duim, B. et al. (1999) Appl. Environ. Microbiol. 65, 2369–75. PubMed ID #10347015.
  Sphingomonas paucimobilis		Yes	Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42. PubMed ID #9464390.
  Flavobacterium species		Yes	Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42. PubMed ID #9464390.

  Bacteria – Gram Positive

  Sample Type	Promega	External	Comments
  Staphylococcus epidermidis	Yes	Yes	Cells need to be treated with a lytic enzyme before lysis buffer addition. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section. Reference: Vandecasteele, S.J. et al. (2001) J. Bacteriol. 183, 7094–101. PubMed ID #11717267.
  Bacillus anthracis		Yes	Pomerantsev, A.P. et al. (2006) Infect. Immun. 74, 682–93. PubMed ID #16369025.
  Bacillus cereus		Yes	van Schaik, W. et al. (2004) J. Bacteriol. 186, 316–25. PubMed ID #14702299.
  Bacillus subtilis		Yes	Ben-Yehuda, S. and Losick, R. (2002) Cell 109, 257–66. PubMed ID #12007411.
  Staphylococcus, 12 strains		Yes	Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6. PubMed ID #12904342.
  Lactobacillus acidophilus		Yes	Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6. PubMed ID #12904342.
  Actinomyces naeslundii		Yes	Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6. PubMed ID #12904342.
  Streptococcus pneumoniae		Yes	Brown, J.S. et al. (2002) Infect. Immun. 70, 4389–98. PubMed ID #12117949.

  Bacteria – Other bacteria

  Sample Type	Promega	External	Comments
  Treponema species		Yes	Cells were grown, centrifuged and washed three times with PBS. Protocol for Gram negative bacteria ("Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria") was then followed. Additional Resource: Greene, S.R. and Stamm, L.V. (1998) Promega Notes 68, 30.
  Methanococcus jannaschii		Yes	Löwe, J. and Amos, L.A. (1998) Nature 391, 203–6. PubMed ID #9428770.
  Methanosarcina mazei		Protocols Complete Protocol Wizard® Genomic DNA Purification Kit Technical Manual PDF (365 KB) Quick Protocols Wizard Genomic DNA Purification Kit Quick Protocol FB022 PDF (214 KB) Certificate of Analysis Lookup Certificate of Analysis Storage Conditions +15C TO +30C Use Restrictions For Research Use Only. Not for Use in Diagnostic Procedures. Resources Articles Calculating Nucleic Acid Samples or Protein Concentration Using GloMax-Multi Microplate Instrument Manual and Automated Genomic DNA Purification Methods for Genotyping Arrays Isolation of gDNA From Small Volumes of Whole Blood Using the Wizard Genomic DNA Kit Citations In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection -- 2017 PLos ONE Other DNA Purification (An overview)