XhoI

Description

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• Overview
• Protocols
• Specifications
• Resources

• Cut Site:

  C^?TCGA  G
  G  AGCT_?C

  Usage Note: Ends generated with XhoI can be directly ligated to ends generated by SalI. Neither recognition site is regenerated in the ligation product.

  Incubation Conditions: Buffer D. 37°C.

  Source: Xanthomonas holcicola.

  Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM KCl, 0.1% Triton® X-100, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

  Percent Activity in 4-CORE® Buffer:

  A	B	C	D	MULTI-CORE™
  25–50%	75–100%	75–100%	100%	10–25%

  Frequency of Cutting:

  ?	Ad-2	?X174	pUC18	M13mp18	pBR322
  1	6	1	0	0	0

• Protocols

  Complete Protocol

  

  Restriction Enzyme Protocol

  PDF (410 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  -30C TO -10C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Other

  • Restriction Enzyme Resource