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xhoi

XhoI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources

  • Cut Site:

    C?TCGA  G
    G  AGCT?C

    Usage Note: Ends generated with XhoI can be directly ligated to ends generated by SalI. Neither recognition site is regenerated in the ligation product.

    Incubation Conditions: Buffer D. 37°C.

    Source: Xanthomonas holcicola.

    Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM KCl, 0.1% Triton® X-100, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    25–50% 75–100% 75–100% 100% 10–25%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    1 6 1 0 0 0

  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other