GoTaq Hot Start Polymerase

by Promega
Available SKUs: M5001 | M5005 | M5006 | M5008 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Performance and Sensitivity of GoTaq® DNA Polymerase with Built-In Hot Start

    GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase also exhibits 5´?3´ exonuclease activity. The enzyme is supplied with a tube of 25mM MgCl2 to optimize the magnesium concentration in your reactions.

    GoTaq® Hot Start Polymerase is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

    pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

    GoTaq® Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.
    GoTaq® Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.
  • Protocols

    Complete Protocol

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    GoTaq® Hot Start Polymerase Product Information

    PDF (207 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles

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