ReliaPrep™ DNA Clean-Up and Concentration System

by Promega
Available SKUs: A2891 | A2892 | A2893 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Prepare DNA samples for downstream applications

    The ReliaPrep™ DNA Clean-Up and Concentration System is designed to quickly concentrate and purify dilute DNA solutions, extract and purify DNA fragments of 100bp–10kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE) buffer, or to purify products directly from a PCR amplification. This membrane-based system can bind up to 60µg of DNA and concentrate as much as 300µl of dilute DNA, recovering isolated DNA fragments or PCR products in as little as 10 minutes, depending on the number of samples processed and the protocol used.

    A single reagent stream is used for all three procedures, making the system both fast and easy. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, next-generation sequencing or in vitro transcription/translation.

    The ReliaPrep™ DNA Clean-Up and Concentration System can be used with linear DNA fragments, supercoiled plasmid DNA or single-stranded linear or circular DNA.

    Recovery and concentration of DNA from dilute solution using the ReliaPrep™ DNA Clean-Up and Concentration System

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    15016ma-w

    DNA (Cat.# G2101) was diluted in triplicate into a final 100µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Average yield and purity was 90.8%. Samples were further analyzed on an Agilent Bioanalyzer® 2100 using a DNA 1000 chip and protocol.

  • Specifications

    You are viewing: A2891 Change Configuration

    What's in the box?

    Item Part # Size Available Separately

    Collection Tubes

    A130C 1 × 10/pk
    ReliaPrep™ Minicolumns A207A 1 × 10/pack

    Column Wash Solution (CWE)

    A254A 1 × 1ml

    Buffer B (BWB)

    A288A 1 × 11.8ml

    Membrane Binding Solution

    A930A 1 × 4ml View Product

    Elution Tubes

    Z200A 2 × 5/pk

    Nuclease-Free Water

    P119A 1 × 1,250μl View Product

    Certificate of Analysis

    See all certificates for A2891

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

    Storage Conditions

    DD

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