T Cell Activation Bioassay (NFAT), Propagation Model
Assays for the Discovery and Development of Novel Biologics Such as Bispecific Antibodies
The T Cell Activation Bioassays are bioluminescent cell-based assays that overcome the limitations of existing assays and can be used for the discovery and development of novel biologics such as bispecific antibodies and CAR-T cell therapies. The assays consist of a genetically engineered Jurkat T cell line that expresses a luciferase reporter (TCR/CD3 Effector Cells) driven by either an NFAT-response element (NFAT-RE) or an IL-2 promoter.
When the TCR/CD3 Effector Cells (NFAT) are engaged with an appropriate TCR/CD3 ligand or anti-TCR/CD3 antibody, the TCR transduces intracellular signals resulting in NFAT-RE-mediated luminescence. Similarly, when the TCR/CD3 Effector Cells (IL-2) are co-engaged with an anti-TCR/CD3 and an anti-CD28 stimulus, receptor-mediated signaling results in IL-2 promoter-mediated luminescence.
T Cell Activation Bioassay, Propagation Model, allows propagation and banking of the TCR/CD3 Effector Cells. Bio-Glo™ Luciferase Assay System (Cat.# G7940, G7941) is the required reagent for use with T Cell Activation Bioassay, Propagation Model.
T Cell Activation Bioassay (NFAT), Propagation Model, Technical Manual
PDF (2 MB)
T Cell Activation Bioassay (IL-2), Propagation Model Technical Manual
PDF (2 MB)
Certificate of AnalysisLookup Certificate of Analysis
For Research Use Only. Not for Use in Diagnostic Procedures.