Wizard Genomic DNA Purification Kit

by Promega
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Simple Method to Isolate Genomic DNA from Many Sample Types

    The Wizard® Genomic DNA Purification Kit provides a simple, solution-based method for isolation of DNA from white blood cells, tissue culture cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative bacteria. DNA purified with this system is suitable for a variety of applications, including amplification, digestion with restriction endonucleases and membrane hybridizations (e.g., Southern and dot/slot blots).

    Learn more about our custom options for this product at: www.promega.com/custom/

    Sample Types Processed with the Wizard® Genomic Kit

    Blood
    Sample Type Promega External Comments
    Blood and blood cells
    Human whole blood Yes Yes Protocols are provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050 for samples of up to 10ml blood or in 96-well plates ("Isolating Genomic DNA from Whole Blood" sections). The protocol works with the following anticoagulants: EDTA, heparin and citrate.
    Reference:
    Rizzo, W.B., Carney, G. and Lin, Z. (1999) Am. J. Hum. Genet. 65, 1547–60. PubMed ID #10577908
    Mouse whole blood Yes A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050.
    Human peripheral blood mononuclear cells Yes Tan, A. et al. (2006) Clin. Chem. 52, 2250–7.
    PubMed ID #17040960
    Bovine whole blood Yes Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
    PubMed ID #12711348
    Horse whole blood Yes Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
    PubMed ID #12711348
    Sheep whole blood Yes Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
    PubMed ID #12711348
    Antelope whole blood Yes Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
    PubMed ID #12711348
    Dog whole blood Yes Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
    PubMed ID #12711348
    Cat whole blood Yes Walker, J.A. et al.(2003) Anal. Biochem. 316, 259–69.
    PubMed ID #12711348
    Rabbit whole blood Yes Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
    PubMed ID #12711348
    Mammalian Tissues
    Sample Type Promega External Comments
    Mammalian tissues
    Mouse liver Yes Yes Tissue needs to be homogenized prior to isolation. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
    Reference:
    Barazzoni, R. et al. (2005) Endocrinology 146, 2098–106.
    PubMed ID #15618355.
    Mouse tail Yes Yes Tail needs to be treated with proteinase K before purification. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
    Reference:
    Nielsen, J.V. et al. (2007) Development 134, 1133–40.
    PubMed ID #17301088.
    Mouse brain Yes Sinz, E.H. et al. (1999) J. Clin. Invest. 104, 647–56.
    PubMed ID #10487779.
    Mouse Lymphoma Yes Yu, Z. et al. (2006) Cancer Res. 66, 755–62.
    PubMed ID #16424006.
    Rat skeletal muscle, liver and heart Yes Barazzoni, R. et al. (2000) J. Biol. Chem. 275, 3343–7.
    PubMed ID #10652323.
    Human bone marrow aspirate Yes Yu, Z. et al. (2006) Cancer Res. 66, 755–62.
    PubMed ID #16424006.
    Human liver and testicle Yes Maduro, M.R. et al. (2003) Mol. Hum. Reprod. 9, 61–8.
    PubMed ID #12569174.
    Human colon Yes Tost, J. et al. (2003) Nucleic Acids Res. 31, e50.
    PubMed ID #12711695.
    Plant Tissues
    Sample Type Promega External Comments
    Plant tissues
    Tomato leaf Yes Leaf needs to be frozen in liquid nitrogen and ground with a mortar and pestle prior to adding lysis buffer. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Plant Tissue" section.
    Arabidopsis Yes Vorwerk, S. (2001) Wizard® Genomic DNA Purification Kit and the isolation of plant genomic DNA. eNotes
    Maize Yes Vorwerk, S. (2001) Wizard® Genomic DNA Purification Kit and the isolation of plant genomic DNA. eNotes
    Lemna minor (aquatic plant) Yes Stout, L.M. and Nüsslein, K. (2005) Appl. Environ. Microbiol. 71, 2484–92.
    PubMed ID #15870338.
    Medicago truncatula Yes Total DNA was isolated from roots, which may include various mycorrhizal fungi.
    Kosuta, S. et al. (2003) Plant Physiol. 131, 952–62.
    PubMed ID #12644648.
    Tetrahymena thermophila Yes Followed plant DNA protocol ("Isolating Genomic DNA from Plant Tissue"), but skipped freezing in liquid nitrogen and grinding with a mortar and pestle.
    Jacobs, M.E. et al. (2006) Eukaryot. Cell 5, 1990–2000.
    PubMed ID #17012537.
    Cultured Cells
    Sample Type Promega External Comments
    Cultured cells (Established cell lines are not listed.) Yes Yes A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
    Reference:
    Zhang, S. et al. (2006) Cancer Res. 66, 8847–57.
    PubMed ID #16951202.
    Drosophila D.Mel-2 embryonic cells Yes Roberti, M. et al. (2006) Nucleic Acids Res. 34, 2109–16.
    PubMed ID #16648357.
    Mouse embryonic stem cells Yes Oka, M. et al. (2006) J. Biol. Chem. 281, 9901–8.
    PubMed ID #16439359.
    Monkey primary fibroblasts, virus-infected Yes Cells were lysed using 300 µl of Nuclei Lysis Solution with proteinase K and incubating overnight at 37°C.
    Mansfield, K.G. et al. (1999) J. Virol. 73, 10320–8.
    PubMed ID #10559350.
    Vero cells infected with HSV-1 Yes Both viral and genomic DNA were isolated.
    Bower, J.R. et al. (1999) J. Virol. 73, 3843–53.
    PubMed ID #10196279.
    CD45RA+ T cells from cord blood Yes Samples of 2–4 × 106 cells were pelleted, washed with PBS, snap frozen in liquid nitrogen and frozen at –70°C prior to using the Wizard® Genomic DNA Purification Kit. Soares et al. (1998) J. Immunol. 161, 5909–17.
    PubMed ID #9834071.
    Bacteria – Gram Negative
    Sample Type Promega External Comments
    Bacteria – Gram Negative
    Enterobacter cloacae Yes A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
    Escherichia coli Yes Yes A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
    Reference:
    Zheng, W. et al. (2001) EMBO J. 20, 1164–72.
    PubMed ID #11230139.
    Nitrobacter winogradskyi Yes Starkenburg, S.R. et al. (2006) Appl. Environ. Microbiol. 72, 2050–63.
    PubMed ID #16517654.
    Yersinia pestis Yes Flashner, Y. et al. (2004) Infect. Immun. 72, 908–15.
    PubMed ID #14742535.
    Burkholderia species Yes Moore, R.A. et al. (2004) Infect. Immun. 72, 4172–87.
    PubMed ID #15213162.
    Salmonella enterica Yes Nair, S. et al. (2004) J. Bacteriol. 186, 3214–23.
    PubMed ID #15126484.
    Field bacteria samples, non-Agrobacterium Yes Weller, S.A. et al. (2004) Appl. Environ. Microbiol. 70, 2779–85.
    PubMed ID #15128532.
    Haemophilus influenzae Yes Ottemann, K.M. and Lowenthal, A.C. (2002) Infect. Immun. 70, 1984–90.
    PubMed ID #11895962.
    Bordetella bronchiseptica Yes Brockmeier, S.L. et al. (2002) Infect. Immun. 70, 481–90.
    PubMed ID #11796573.
    Leptospira kirschneri Yes Matsunaga, J. et al. (2002) Infect. Immun. 70, 323–34.
    PubMed ID #11748198.
    Klebsiella pneumoniae Yes Yigit, H. et al. (2001) Antimicrob. Agents Chemother. 45, 1151–61.
    PubMed ID #11257029.
    Marine Geobacteraceae Yes Bacteria colonizing energy-harvesting anodes were scraped off, organically extracted and purified using the Wizard® Genomic DNA Purification Kit.
    Bond, D.R. et al. (2002) Science 295, 483–5.
    PubMed ID #11799240.
    Serratia marcescens Yes Peccia, J. and Hernandez, M. (2001) Appl. Environ. Microbiol. 67, 4225–32.
    PubMed ID #11526027.
    Borrelia burgdorferi Yes Chaconas, G. et al. (2001) EMBO J. 20, 3229–37.
    PubMed ID #11406599.
    Campylobacter jejuni Yes Dorrell, N. et al.(2001) Genome Res. 11, 1706–15.
    PubMed ID #11591647.
    Desulfovibrio desulfuricans Yes Rapp-Giles, B.J. et al. (2000) Appl. Environ. Microbiol. 66, 671–7.
    PubMed ID #10653734.
    Vibrio cholerae Yes Stine, O.C. et al. (2000) Infect. Immun. 68, 7180–5.
    PubMed ID #11083852.
    Vibrio harveyi Yes Zhou, W. et al. (1999) Biochemistry 38, 16246–52.
    PubMed ID #10587447.
    Paracoccus denitrificans Yes Shearer, N. et al. (1999) J. Bacteriol. 181, 6907–13.
    PubMed ID #10559155.
    Campylobacter species Yes Before genomic DNA isolation, the bacteria were washed in TE buffer and resuspended in 1ml of TE buffer at ~1 × 109 cells/ml.
    Duim, B. et al. (1999) Appl. Environ. Microbiol. 65, 2369–75.
    PubMed ID #10347015.
    Sphingomonas paucimobilis Yes Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42.
    PubMed ID #9464390.
    Flavobacterium species Yes Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42.
    PubMed ID #9464390.
    Bacteria – Gram Positive
    Sample Type Promega External Comments
    Staphylococcus epidermidis Yes Yes Cells need to be treated with a lytic enzyme before lysis buffer addition. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
    Reference:
    Vandecasteele, S.J. et al. (2001) J. Bacteriol. 183, 7094–101.
    PubMed ID #11717267.
    Bacillus anthracis Yes Pomerantsev, A.P. et al. (2006) Infect. Immun. 74, 682–93.
    PubMed ID #16369025.
    Bacillus cereus Yes van Schaik, W. et al. (2004) J. Bacteriol. 186, 316–25.
    PubMed ID #14702299.
    Bacillus subtilis Yes Ben-Yehuda, S. and Losick, R. (2002) Cell 109, 257–66.
    PubMed ID #12007411.
    Staphylococcus, 12 strains Yes Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
    PubMed ID #12904342.
    Lactobacillus acidophilus Yes Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
    PubMed ID #12904342.
    Actinomyces naeslundii Yes Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
    PubMed ID #12904342.
    Streptococcus pneumoniae Yes Brown, J.S. et al. (2002) Infect. Immun. 70, 4389–98.
    PubMed ID #12117949.
    Bacteria – Other bacteria
    Sample Type Promega External Comments
    Treponema species Yes Cells were grown, centrifuged and washed three
    times with PBS. Protocol for Gram negative bacteria ("Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria") was then followed.
    Additional Resource:
    Greene, S.R. and Stamm, L.V. (1998) Promega Notes 68, 30.
    Methanococcus jannaschii Yes Löwe, J. and Amos, L.A. (1998) Nature 391, 203–6.
    PubMed ID #9428770.
    Methanosarcina mazei
  • Protocols

    Complete Protocol

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  • Wizard® Genomic DNA Purification Kit Technical Manual

    PDF (365 KB)

    Quick Protocols

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    Wizard Genomic DNA Purification Kit Quick Protocol FB022

    PDF (214 KB)

  • Certificate of Analysis

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    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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