PowerPlex Fusion System
Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more at: www.promega.com/products/genetic-identity/forensic-grade-faq/. The PowerPlex Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. This five-color system allows co-amplification and fluorescent detection of the 13 core CODIS (US) loci (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the 12 core European Standard Set loci (TH01, vWA, FGA, D21S11, D3S1358, D8S1179, D18S51, D10S1248, D22S1045, D2S441, D1S1656 and D12S391) and Amelogenin for gender determination. In addition, the male-specific DYS391 locus is included to identify null Y allele results for Amelogenin. The Penta D, Penta E, D2S1338 and D19S433 loci are included to increase discrimination and allow searching of databases that include profiles with these popular loci. This extended panel of STR markers is intended to satisfy both CODIS and ESS recommendations. The PowerPlex Fusion System works well with extracted DNA samples, including low amounts of template DNA, mixtures and inhibitor-laden samples. The PowerPlex Fusion System also is compatible with direct amplification, enabling streamlined STR databasing efforts. Amplification can be successfully performed with sample types such as FTA card punches as well as pretreated swabs, Bode Buccal DNA Collector punches or S&S 903 punches. Fast cycling conditions used with the PowerPlex Fusion System reduce sample-processing time for all samples. The PowerPlex Fusion System is compatible with the ABI PRISM 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper ID and ID-X software and are available for download. The PowerPlex Fusion System was given NDIS approval in July 2011 for NDIS CODIS databasing.