TMB Stabilized Substrate for Horseradish Peroxidase
TMB Stabilized Substrate is a stable, ready-to-use TMB (3,3´, 5,5´-tetramethylbenzidine) color development substrate for localization of horseradish peroxidase-conjugated antibodies on dot blots and Western blots. It is easier to use than 4-chloro-1-naphthol (CN), which must be prepared immediately before use. TMB Stabilized Substrate comes premixed and fully diluted in a proprietary buffer containing less than 0.5% organic solvent.
TMB Stabilized Substrate is at least threefold more sensitive than 4-chloro-1-naphthol (CN); as little as 412pg of ?-galactosidase detected on TMB blot as compared to 1.12ng on CN blot when detected with a ?-galactosidase-specific antibody and HRP-conjugated secondary antibody. The color is much more stable than 4-chloro-1-naphthol and photographs more easily.
Note: Not recommended for use with ELISAs.References
- Larson, G. (1992) Promega Notes 39, 17–18.
- Cullinane, A. et al. (2007) Diagnosis of equine infectious anaemia during the 2006 outbreak in Ireland. Vet. Rec. 161, 647–52.
- Tetteh, K.K.A. et al. (2005) Extensive antigenic polymorphism within the repeat sequence of the Plasmodium falciparum merozoite surface protein 1 block 2 is incorporated in a minimal polyvalent immunogen. Infect. Immun. 73, 5928–35.
- Stenbak, C.R. and Linial, M.L. (2004) Role of the C terminus of foamy virus Gag in RNA packaging and pol expression. J. Virol. 78, 9423–30.
- Mello, K. et al. (2002) A multigene family that interacts with the amino terminus of plasmodium MSP-1 identified using the yeast two-hybrid system. Eukaryot. Cell 1, 915–25.
Certificate of AnalysisLookup Certificate of Analysis
+15C TO +30C
For Research Use Only. Not for Use in Diagnostic Procedures.