TRKC Kinase Enzyme System
Recombinant human TRKC (amino acids 507-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. TRKC is a member of the TRK family of tyrosine kinase receptors and is the high affinity catalytic receptor for the neurotrophin NT-3 (neurotrophin-3). TRKC mediates the multiple cellular effects of the NT-3 neurotrophic factor, which includes neuronal differentiation and survival. TRKC has been implicated in insulin signaling pathway through interactions with the MUSK protein receptor and the VEGF receptor. Mutations in the TRKC gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.