MSI Analysis System, Version 1.2 - MyBio Ireland - Promega

MSI Analysis System, Version 1.2

The MSI Analysis System, Version 1.2, is a fluorescent multiplex PCR-based method for detection of microsatellite instability (MSI), a form of genomic instability. This instability is due to either insertion or deletion of repeating units during DNA replication and failure of the mismatch repair system (MMR) to correct these errors. MSI analysis typically involves comparison of allelic profiles of microsatellite markers generated by amplification from matching pairs of test samples, which may be MMR-deficient, and normal tissue samples. New alleles in the abnormal sample not found in the corresponding normal sample indicate the presence of MSI. MSI analysis can be used as a screening method to identify samples for further characterization. The MSI Analysis System, Version 1.2, includes fluorescently labeled primers (marker panel) for co-amplification of seven markers for analysis of the MSI-high (MSI-H) phenotype, including five nearly monomorphic mononucleotide repeat markers (BAT-25, BAT-26, MONO-27, NR-21 and NR-24) and two highly polymorphic pentanucleotide repeat markers (Penta C and Penta D). Amplified fragments are detected using ABI 3130 or 3500 series Genetic Analyzers (as well as other legacy instruments) after spectral calibration. Panels and bins text files simplify and standardize data analysis by providing automated assignment of genotypes using GeneMapper software.

Ask a Question or Request a Quote

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.