GoTaq 2-Step RT-qPCR System
The GoTaq qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye. The master mix contains BRYT Green dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR Green I. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq Hot Start Polymerase, MgCl2, dNTPs, BRYT Green Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included. The GoTaq 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript Reverse Transcription System and GoTaq qPCR Master Mix. The GoScript Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions. The GoTaq 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript Reverse Transcriptase and GoTaq qPCR Master Mix in a single-step real-time amplification reaction. The GoScript RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript Reverse Transcriptase, RNasin Plus RNase Inhibitor and additives to enhance single-step reactions.