GoTaq G2 DNA Polymerase
The second generation of GoTaq products, GoTaq G2 DNA Polymerase reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency. GoTaq G2 DNA Polymerase is supplied with 5X Green GoTaq Reaction Buffer and 5X Colorless GoTaq Reaction Buffer. Both buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction. GoTaq G2 DNA Polymerase is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The enzyme is a full-length form of Taq DNA polymerase that exhibits 5'-->3' exonuclease activity. The 5X Green GoTaq Reaction Buffer contains two dyes (blue and yellow) that separate during electrophoresis to indicate migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.