GoTaq Green Master Mix
GoTaq DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq DNA polymerase. For ultimate convenience, choose GoTaq Colorless Master Mix or GoTaq Green Master Mix and directly substitute in your current PCR application—no need to change cycling parameters. Both Master Mixes are ready-to-use 2X solutions that contain GoTaq DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3-5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp). GoTaq Colorless Master Mix has the same formulation as the GoTaq Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq Master Mixes have sufficient density for direct loading onto agarose gels. Use the green master mix for direct-to-gel analysis after amplification and the colorless master mix for post-amplification analysis by fluorescence or absorbance without prior DNA purification. Reactions can be set up in less than a minute; simply add your template, water and primers and go! GoTaq Green and Colorless Master Mix reaction size is based on a 50ul reaction volume.