Wizard Plus Midipreps DNA Purification System
The resin-based Wizard Plus Midipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. When using the standard protocol, the entire midiprep process can be completed in 90 minutes or less, yielding up to 200ug of high-quality DNA with no organic extractions or ethanol precipitations. Multiple midipreps can be easily processed at one time with the Vac-Man (20-sample capacity, Cat.# A7231) or Vac-Man Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard Midicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing or restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511). The system includes sufficient reagents for 25 DNA isolations from 10-100ml of liquid culture.