Lys-C, Mass Spec Grade, and Lys-N, Mass Spec Grade

by Promega
Available SKUs: VA1170 | VA1180 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Active Under Strong Denaturing Conditions

    Endoproteinase Lys-C, Mass Spec Grade, is a highly-purified serine protease that hydrolyzes specifically at the carboxyl side of lysines. Lys-C retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins. Lys-C, Mass Spec Grade, has optimal activity in the range of pH 7.0—9.0. This protease can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. 

    Endoproteinase Lys-N, Mass Spec Grade, is a zinc metalloprotease that cleaves at the amino side of lysines. Lys-N, Mass Spec Grade, retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins. Charged amino-terminal peptide fragments generated by Lys-N, Mass Spec Grade, are useful for de novo sequencing with ETD fragmentation techniques.

    Digestion Under Strong Denaturing Conditions

    Image of digestion under strong denaturing conditions.
    Panel A. One-step digestion under strong denaturing conditions. Panel B. Two-step digestion using strong denaturing conditions for the first step and diluting for trypsin digestion in the second step.

    Native Lys-C Interchangeable with Recombinant Lys-C

    Comparison of recombinant and native Lys-C digestions.
    Comparison of lysine-specific cleavages. Mass Spec-Compatible Yeast Protein Extract, Intact (Cat.# V7341) was digested with either rLys-C (P. aeruginosa; Cat.# V1671) or native Lys-C (Lysobacter sp.; Cat.# VA1170)) at a ratio of 1:100.
  • Item Part # Size

    Lys-C Mass Spec Grade

    VA117A 1 × 20μg
    See all certificates for VA1170 See all certificates for VA1170

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

    Storage Conditions

    BB

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