NanoBRET Positive Control
The NanoBRET Positive Control Vector encodes a NanoLuc-HaloTag fusion protein that tethers together the NanoLuc donor and HaloTag acceptor proteins to ensure efficient energy transfer. The NanoBRET Positive Control Vector should be diluted in Transfection Carrier DNA to reduce cellular expression levels due to the high NanoBRET signal produced. The control vector can be used when performing instrument setup or as a reference control when performing other NanoBRET assays. The NanoBRET Positive Control contains the following key features: 1) NanoLuc-HaloTag fusion gene. 2) Kanamycin resistance gene for selection in bacteria. 3) A CMV promoter for high-level expression of NanoLuc-HaloTag fusion in mammalian cells. The Transfection Carrier DNA contains the following key features: Ampicillin resistance gene for selection in bacteria.