TNT T7 Quick Coupled Transcription/Translation System
Express Protein from PCR Fragments or Plasmid DNA
The TnT® Quick Coupled Transcription/Translation Systems provide convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix.
The TnT® Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2–2.0ug of circular plasmid DNA containing a T7 or SP6 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TnT® Quick Master Mix and incubated in a 50ul reaction volume for 60–90 minutes at 30°C. The expression reaction produces significant quantities of protein for a variety of applications including GST pull-downs and gel shift assays.
These mammalian-based systems express soluble, functional proteins that are post-translationally modified, unlike E. coli-based systems. Expressed proteins can be used directly after expression; no additional protein purification needed.
For more information about cell-free protein expression, see the Protein Expression chapter of the Protocols & Applications Guide.
TnT® Quick Coupled Transcription/Translation System Technical Manual
PDF (502 KB)
TNT Quick Coupled Transcription/Translation System Quick Protocol FB030
PDF (90 KB)
Certificate of AnalysisLookup Certificate of Analysis
LESS THAN -65C
For Research Use Only. Not for Use in Diagnostic Procedures.