Wheat Germ Extract

by Promega
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L4380

Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract preparation include grinding in an extraction buffer followed by centrifugation to remove cell debris and chromatography that removes endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA, thus minimizing background translation.

    The extract contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), spermidine to stimulate the efficiency of chain elongation, magnesium acetate and potassium acetate. Potassium Acetate enhances translation for a wide range of mRNAs. Three Amino Acid Mixtures are provided, which enable use of different radioisotopes.

    For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

    Learn more about our custom options for this product at: www.promega.com/custom/

    Applications
    • Expression of eukaryotic messages that do not express well in reticulocyte lysates
    • Expression of plant mRNAs
    • Expression of mammalian transcription factors
  • Protocols

    Complete Protocol

    Download PDF

    Wheat Germ Extract Technical Manual

    PDF (430 KB)

    Quick Protocols

    Download PDF

    Wheat Germ Extract Quick Protocol FB031

    PDF (84 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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