NanoBRET Ubiquitination Starter Kit
Regulating overall cellular protein homeostasis is critical for maintaining cell health and often altered by cellular treatments or disease states. Most proteins and their abundance are regulated via the ubiquitin proteasome system (UPS), which uses ubiquitin conjugation to signal proteins to be trafficked to the proteasome for degradation. Ubiquitination on any given target can vary in levels, mono- and poly-ubiquitination, and mediated through a variety of amino acid linkages. The NanoBRET Ubiquitination Starter Kit provides the tools to create target-specific live-cell ubiquitin assays that globally measure all types of ubiquitination on a target protein. The assays can be used to measure dynamic increases or decreases in the relative levels of target protein ubiquitination following cellular treatments, such as small molecules or pathway inducers, that would influence protein stabilization or degradation, respectively. These assays can be particularly useful for studying targeted degradation compounds because the bioluminescent resonance energy transfer ratio of the NanoBRET signal means you can investigate proteasome recruitment while simultaneously monitoring target protein levels to assess degradation.