NanoBRET-based protein:protein interaction (PPI) assays are proximity-based assays that use NanoLuc Luciferase as a BRET energy donor and HaloTag protein, labeled with the HaloTag NanoBRET 618 Ligand, as the energy acceptor to measure the interaction of specific protein pairs.
![pGL4.31[luc2P/Gal4UAS/Hygro] Vector - MyBio Ireland - Promega](http://mybio.ie/cdn/shop/products/c9351.jpg?v=1614104091)