The pFN11A (BIND) Flexi Vector is a component of the CheckMate/Flexi Vector Mammalian Two-Hybrid System. This ampicillin-resistant vector is designed to express a fusion protein comprised of a DNA-binding domain of the yeast GAL4 gene, a linker segment and an in-frame protein-coding sequence, under the control of the human cytomegalovirus (CMV) immediate early promoter. The pFN11A (BIND) Flexi Vector encodes a Renilla luciferase gene which may be used to normalize for transfection differences between samples within an experiment.