ViaFect Transfection Reagent is a novel formulation reagent designed to efficiently introduce DNA into a wide variety of cell lines. ViaFect Transfection Reagent has performed in commonly used adherent cell models and also in cells lines traditionally thought of as difficult to transfect such as suspension cells and stem cell-derived lines. This gentle, low-toxicity reagent allows cells to stay healthy and metabolically active during transfection-based experiments and offers an easy-to-use protocol that does not require removal of serum or culture medium prior to use. After introducing the reagent:DNA complex no washing or medium changes are required. ViaFect Transfection Reagent provides robust performance with minimal optimization allowing simple design of more relevant assays.