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FuGENE® 4K Transfection Reagent

Best-in-class DNA transfection reagent for routine and challenging cell lines

FuGENE® 4K is a 100% synthetic, multicomponent transfection reagent designed for the delivery of DNA into challenging and routine mammalian cell lines. Improving on previous generations of FuGENE® 4K efficiently delivers DNA into a wide range of cell lines while providing the gentle, low-toxicity transfection you've come to expect from FuGENE® reagents.

The FuGENE® 4K advantage

Get the most out of transfection-based experiments. Obtain higher transfection efficiencies that leading competitor reagents while minimizing impact on cell health

Ideal for protein and viral production. FuGENE® 4K is made with animal-free reagents and shows strong performances in HEK293 and CHO cells.

Free up valuable lab time. A quick and easy protocol helps you obtain superior results with minimal optimization.

Achieve High Transfection Efficiency with Minimal Effect on Cell Health

FuGENE® 4K Reagent delivers improved DNA transfection performance while minimizing impact on cell health.

A375, HCT116, CHO-K1 and H1299 cell lines were transfected with a GFP exoression construct using FuGENE® $K or Lipofectamine 3000 transfection reagent (ThermoFisher Scientific) with varying reagents DNA rations. After 48 hours, cells in clear-bottom plate were measured for total GFP fluorescence. Cell viability was measured on a duplicate plate using the CellTiter-GLO Luminescent Cell Viability Assay.