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10X Flexi Enzyme Blend (SgfI & PmeI)

by Promega

The 10X Flexi Enzyme Blend (SgfI and PmeI) is required to screen Flexi Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi Vectors or transfer protein-coding regions between Flexi Vectors. For more information see the Flexi Vector Systems Technical Manual #TM254. Recognition Sites: SgfI recognition site: 5…GCGATtCGC…3 3…CGCsTAGCG…5. PmeI recognition site: 5…GTTTtAAAC…3 3…CAAAsTTTG…5. Storage Buffer: The 10X Flexi Enzyme Blend (SgfI and PmeI) is supplied in 10mM Tris-HCl (pH 7.4 at 25°C), 150mM KCl, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.35mg/ml BSA, 0.05% Thesit and 50% glycerol. 5X Flexi Digest Buffer: The composition of 5X Flexi Digest Buffer is 50mM Tris-HCl (pH 7.9 at 37°C), 50mM MgCl2, 250mM NaCl, 5mM DTT, 0.5mg/ml acetylated BSA.