The DNA Step Ladders are ladders of defined sizes with exact incremental steps between bands. The ladders are not intended for use in quantitative analysis. Each ladder is provided with a tube of 6X Blue/Orange Loading Dye. The fragments may be stained with ethidium bromide. 50bp DNA Step Ladder: Sixteen DNA fragments ranging from 50bp to 800bp in 50bp increments plus an 1,800bp backbone fragment. All bands except the 800bp band are of equal intensity; the 800bp band is ~3 times more intense. Recommended loading: 5microl/lane.