aAPC/CHO-K1 Cells, Propagation Model

The antibodies used to stimulate T cell activation via interaction with co-stimulatory receptors on the effector cells can be crosslinked via binding to FcgammaRIIb receptors on aAPC/CHO-K1 cells. Based on the properties of the antibody tested, the Co-Stimulatory Immune Checkpoint (i.e., ICOS or CD28) bioassays can be conducted in a single genetically engineered cell system or dual genetically engineered cell systems.FcgammaRIIb aAPC/CHO-K1 Cells are engineered to express human FcgammaRIIb and aAPC provided in cryopreserved Cell Propagation Model (CPM) and thaw-and-use formats. The CPM cells can be thawed, propagated and banked for long-term use. These are not reporter cells, and they function to provide activation of the TCR/CD3 complex on effector cells and crosslink anti-CD28 and anti-ICOS agonist antibodies.aAPC/CHO-K1 Cells are engineered to express aAPC provided in cryopreserved Cell Propagation Model (CPM) and thaw-and-use formats. The CPM cells can be thawed, propagated and banked for long-term use. These are not reporter cells, and they function to provide activation of the TCR/CD3 complex on effector cells. These cells do not express FcgammaRIIb.