| Immunogen This antibody was raised against a phospho-peptide corresponding to phospho-Histone H2AX (Ser139). Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. AbFlex® Histone H2A.XS139ph antibody (rAb) tested by Western blot. AbFlex® AbFlex® Histone H2A.XS139ph antibody (rAb) tested by Western blot. 15 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex® Histone H2A.XS139ph antibody at 0.5 µg/ml. Background Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation |
