RAP1 / TERF2IP antibody (pAb)

by Active Motif
Available SKUs: 61069 |
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for RAP1 / TERF2IP antibody (pAb)Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

for RAP1 / TERF2IP antibody (pAb)Immunogen

This RAP1 / TERF2IP antibody was raised against a peptide within human RAP1 / TERF2IP.

for RAP1 / TERF2IP antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RAP1 / TERF2IP antibody (pAb)References

RAP1 / TERF2IP antibody (pAb) tested by immunoprecipitation.

RAP1 / TERF2IP antibody (pAb) tested by Immunoprecipitation.
10 µl of RAP1 / TERF2IP antibody was used to immunoprecipitate RAP1 / TERF2IP from 250 µg of K-562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the RAP1 / TERF2IP antibody at a dilution of 1:500.

RAP1 / TERF2IP antibody (pAb) tested by Western blot.

RAP1 / TERF2IP antibody (pAb) tested by Western blot.
Nuclear extract of K-562 cells (30 µg) probed with RAP1 / TERF2IP antibody (pAb) at a dilution of 1:500.

for RAP1 / TERF2IP antibody (pAb)Background

RAP1 / TERF2IP (Repressor/activator protein 1, Telomeric repeat-binding factor 2-interacting protein 1) is a protein associated with telomeres that acts both as a modulator of telomere function and as a regulator of transcription. RAP1 is a component of the shelterin (telosome) protein complex and is involved in the regulation of telomere length and serves to protect telomeric repeats from degradation. In contrast to other components of the shelterin complex, RAP1 is dispensable for telomere end-capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. RAP1 is recruited to telomeric repeats via its interaction with TERF2. Independent of its function in telomeres, RAP1 also acts as a transcriptional regulator. It is recruited to non-telomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. RAP1 is also present in the cytoplasm and associates with the I?B-kinase (IKK) complex and acts as a regulator of the NF?B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activation of the expression of NF?B target genes.

for RAP1 / TERF2IP antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for RAP1 / TERF2IP antibody.

for RAP1 / TERF2IP antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RAP1 / TERF2IP antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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