MED1 antibody (pAb), sample
for MED1 antibody (pAb)Application Notes
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution
for MED1 antibody (pAb)Immunogen
This MED1 antibody was raised against a peptide within the C-terminal region of human MED1.
for MED1 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for MED1 antibody (pAb)References
MED1 (Mediator complex subunit 1, TRIP2, Thyroid receptor-interacting protein 2) is a component of the Mediator complex, a coactivator involved in the regulated transcription of most genes transcribed by RNA polymerase II. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. MED1 is involved in supporting hematopoietic stem and/or progenitor cell function.
for MED1 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for MED1 (TRIP2) antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for MED1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting