FancD2 antibody (pAb)

by Active Motif
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Description

for FancD2 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:5,000 dilution

for FancD2 antibody (pAb)Immunogen

This FancD2 antibody was raised against a peptide within the internal region of human FancD2.

for FancD2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for FancD2 antibody (pAb)References

 
FancD2 antibody (pAb) tested by immunoprecipitation.

FancD2 antibody (pAb) tested by Immunoprecipitation.
10 µl of FancD2 antibody was used to immunoprecipitate FancD2 from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FancD2 antibody at a dilution of 1:500.

FancD2 antibody (pAb) tested by Western blot.

Western blot of FancD2 antibody.
Lane 1: HeLa whole-cell extract (40 µg). Lane 2: Nuclear extract (40 µg) of K-562 cells. Both probed with FancD2 antibody (pAb) at a 1:500 dilution.

for FancD2 antibody (pAb)Background

FancD2 (Fanconi anemia complementation group D2) is a protein encoded by a gene mutated in Fanconi anemia, a recessive genetic disorder characterized by chromosomal instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. FancD2 is monoubiquitinated in response to DNA damage and is required for maintenance of chromosomal stability and the repair of DNA double-strand breaks. FancD2 also likely plays a role in activating the S-phase and G2/M DNA damage checkpoints. It promotes the loading of BRCA2 and FANCD1 onto damaged chromatin and may also be involved in B-cell immunoglobulin isotype switching.

for FancD2 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for FancD2 antibody.

for FancD2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for FancD2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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