BCL11A / CTIP1 antibody (pAb), sample

by Active Motif
Available SKUs: 61052 |
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for BCL11A / CTIP1 antibody (pAb)Application Notes

Validated Applications:
IP: 5 - 10 µl per IP
WB: 1:1,000 - 1:5,000 dilution

for BCL11A / CTIP1 antibody (pAb)Immunogen

This BCL11A / CTIP1 antibody was raised against a peptide within the N-terminal region of human BCL11A / CTIP1.

for BCL11A / CTIP1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BCL11A / CTIP1 antibody (pAb)References

BCL11A / CTIP1 antibody (pAb) tested by immunoprecipitation.

BCL11A / CTIP1 antibody (pAb) tested by Immunoprecipitation.
10 µl of BCL11A / CTIP1 antibody was used to immunoprecipitate BCL11A / CTIP1 from 250 µg of Raji nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the BCL11A / CTIP1 antibody at a dilution of 1:1,000.

BCL11A / CTIP1 antibody (pAb) tested by Western blot.

BCL11A / CTIP1 antibody (pAb) tested by Western blot.
Nuclear extract of Raji cells (20 µg per lane) probed with BCL11A / CTIP1 antibody at a dilution of 1:1,000.

for BCL11A / CTIP1 antibody (pAb)Background

BCL11A / CTIP1 (B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1) is a transcriptional regulator, a myeloid and B-cell proto-oncogene and plays important roles in leukemogenesis and hematopoiesis. BCL11A / CTIP1 is involved in regulating hemoglobin gene expression. BCL11A regulates hemoglobin gene switching during fetal development and silencing of ?-globin expression in adults. BCL11A interacts with the Mi-2/NuRD chromatin remodeling complexes, as well as the erythroid transcription factors GATA1 and FOG1. Abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells.

for BCL11A / CTIP1 antibody (pAb)Positive Control

Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for BCL11A / CTIP1 antibody.

for BCL11A / CTIP1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BCL11A / CTIP1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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