ERR-? antibody (pAb)

by Active Motif
Available SKUs: 61047 |
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for ERR-a antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

for ERR-a antibody (pAb)Immunogen

This ERR-alpha antibody was raised against a peptide within the C-terminal region of human ERR alpha.

for ERR-a antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ERR-a antibody (pAb)References

ERR-? antibody (pAb) tested by Western blot.

Western blot of ERR-? antibody.
Nuclear extract of MDA-MB-231 cells (20 µg per lane) probed with ERR alpha antibody at a dilution of 1:500.

for ERR-a antibody (pAb)Background

ERR-? (Estrogen-related receptor alpha, NR3B1) is an orphan nuclear receptor similar to the Estrogen Receptor. It is a sequence-specific DNA binding transcriptional activator that is constitutively active in the absence of ligand binding. No true physiological ligands have been found, but estrogen-like compounds from plants have been shown to activate ERR-?. ERR-? and ER-? have been found to regulate an overlapping set of genes, and ERR-? appears to modulate the activity of ER-? in some tissue types. ERR-? regulates genes involved in mitochondrial biogenesis, gluconeogenesis, oxidative phosphorylation, and fatty acid metabolism. ERR-? knockout mice display impaired fat metabolism and absorption.

for ERR-a antibody (pAb)Positive Control

Active Motif's MDA-MB-231 nuclear extract (Catalog No. 36080) can be used as a positive control for ERR-? antibody.

for ERR-a antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ERR-a antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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