The HAT Assay Kit includes enough recombinant human p300 to screen 96 wells of inhibitor compounds, and anacardic acid is included for use as a control, as it is a potent HAT inhibitor. All assay buffers have been thoroughly tested to ensure that no components interfere with either activity or inhibition assays. Histone H3 and Histone H4 N-terminal peptides are included as HAT substrates, so you can use the one best suited for your HAT enzyme.
Figure 1: p300 activity and inhibition.
The HAT activity of 50 ng of p300 was assayed with 50 µM acetyl-CoA and either 50 µM Histone H3 or Histone H4 peptide as the substrate. Activity was inhibited with 15 µM anacardic acid or 25 µM garcinol.
Contents & Storage
1 x 96 rxns of recombinant p300 protein, catalytic domain; histone H3 and H4 substrate peptides, Acetyl-CoA, 5X Assay Buffer, Developer and Developing & Stop Solutions. A 96-well plate and plate sealer are also included. Recombinant p300 protein, catalytic domain; and Acetyl-CoA are to be stored at -80°C. Store histone H3 and H4 substrate peptides, 5X Assay Buffer, Developer and Developing So