The Hydroxymethyl Collector™ Kits are designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation* for analysis. They utilize a ?-glucosyltransferase enzyme to transfer a modified glucose moiety to 5-hydroxymethylcytosine residues in double-stranded DNA. This modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment with streptavidin magnetic beads.
* Song, C.X., et al., Nature Biotechnology. 29, 68-72 (2011).
Patent US 8,741,567 B2.
Active Motif's Hydroxymethyl Collector™ and Hydroxymethyl Collector™-Seq Kits are currently unavailable.
Please check out our hMeDIP Kit (Catalog No. 55010) as an alternative for the enrichment of DNA fragments containing 5-hydroxymethylcytosine using antibody-based enrichment.