5-Carboxylcytosine DNA Standard
5-Carboxylcytosine (5-caC) is a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
To better understand the role of 5-carboxylcytosine in genomic DNA, Active Motif has created the 5-Carboxylcytosine DNA Standard Kit. This kit includes two double-stranded DNA oligonucleotides, one oligonucleotide contains a total of 12 carboxylcytosine modifications and the other oligonucleotide contains only unmodified cytosine residues. These oligonucleotides can be used as positive and negative controls in the interrogation of 5-carboxylcytosine. To learn more about the 5-Carboxylcytosine DNA Standard Kit, click on the Documents tab below to download our data sheet.