PvuRts1 I restriction enzyme
for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeContents
50 Units PvuRts1 I restriction enzyme
10X PvuRts1 I Reaction Buffer
1 M DTT
for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeApplication Notes
PvuRts1 I restriction enzyme can be used to directly cleave hydroxymethylated DNA in its non-glucosylated form. The PvuRts1 I enzyme also digests ? and ?-glucosylated hydroxymethylcytosine DNA, but at a lower efficiency than the non-glucosylated form. The enzyme is specific to 5-hmC DNA and will not digest 5-methylcytosine residues or unmethylated DNA. The enzyme is best suited for use in regions of high 5-hmC density, such as gene bodies.
for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeProtein Details
PvuRts1 I restriction enzyme recognizes the sequence hmCN11-12/N9-10G.
One unit is defined as the amount of enzyme required to fully cleave 1 µg of 5-hmC DNA Standard (Catalog No. 55008) in 30 minutes at 22°C in a total reaction volume of 30 µl. A pilot titration of enzyme is recommended for cleavage of genomic DNA.
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant PvuRts1 I (PvuRts1I) restriction enzymeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.