Nuclear Complex Co-IP Kit
Co-immunoprecipitation (Co-IP) is often used to study the relationships between nuclear protein complexes and nuclear processes such as intracellular signaling and transcriptional regulation. However, because these interactions are often labile and the proteins & their modifications can be fragile, traditional methods to isolate and study DNA-binding proteins often lead to disruption of nuclear complexes and a loss of protein integrity. Active Motif’s Nuclear Complex Co-IP Kit improves Co-IP by providing a gentle extraction procedure and optimized immunoprecipitation reagents that enhance the recovery of intact nuclear protein complexes.
For a complete list of reagents included in the Nuclear Complex Co-IP kit, please select the Contents tab below. For optimization of your Co-IP conditions, additional 5X IP High Buffer, 5X IP Low Buffer and Detergent may be purchased separately.
Antibody binding beads are NOT included in the Nuclear Complex Co-IP Kit and must be supplied by the user. Active Motif recommends our Protein G Agarose Columns for easy and efficient capture of the IP complex. The Protein G Agarose Columns contain pre-washed protein G agarose beads that have been specifically engineered to reduce non-specific binding. The beads are provided in ready to use filtration columns that streamline the antibody binding, wash and elution steps.
Co-IP of highly pure, intact nuclear protein complexes
- Efficient Protocol: Simple and easy to use
- Accuracy: Extraction procedure preserves nuclear protein complexes without disrupting protein/protein interactions
- Flexibility: Immunoprecipitation reagents enable detection of protein/protein interactions of varying strength
- Convenient: No need for creating fusion constructs or two-hybrid libraries
*TERMS & CONDITIONS: This offer is valid on a single order through March 31, 2018. Offer valid in the United States and Canada and other countries through which Active Motif sells directly. For countries in which Active Motif does not sell directly, please contact your distributor. Offer may not be applied to existing, pending, or prior orders. Cannot be combined with any other promotions or discounts. Limit 1 promotion per order. Promotion code must be referenced when placing your order.