Nucleosome Preparation Kit
Active Motif’s Nucleosome Preparation Kit is designed to prepare mono- and oligonucleosomes starting from cells. Nucleosomes comprise the smallest subunit of chromatin and consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Each nucleosome is separated by a linker of DNA. The Nucleosome Preparation Kit uses an enzymatic cocktail to digest the linker DNA. By adjusting the digestion time, it is possible to control the amount of mononucleosomes or oligonucleosomes extracted. Because isolated nucleosomes are more physiologically relevant than histones alone or synthetic peptides, they are ideally suited for use as substrates in the analysis of histone post-translational modifications, enzyme kinetics or inhibitor screening studies.
Gel shift showing preservation of intact nucleosomes.
- More physiologically relevant substrates than synthetic peptides
- Useful for the analysis of histone post-translational modifications, enzyme kinetics or inhibitor screening studies
- Easily adjust the levels of mononucleosomes or oligonucleosomes isolated by adjusting digestion times