TransAM Nrf2 Kits are DNA-binding ELISAs that provide everything needed to study NF-E2-related Factor 2 (Nrf2), including a positive control extract. The kit can be used with human, mouse and rat extracts.NF-E2-related factor 2 (Nrf2), also known as Nuclear Factor (erythroid-derived 2)-like 2 (NFE2L2) is a critical transcription factor in oxidative stress signaling. Nrf2 is a basic leucine zipper transcription factor that binds to the antioxidant responsive element (ARE) and may serve as a master regulator in cellular defense pathways in protecting a wide variety of tissues from various toxic exposure. Nrf2 is retained in the cytoplasm under normal conditions by interaction with the inhibitor KEAP1, but following oxidative stress Nrf is released from the inhibitor and translocates to the nucleas for activation of ARE-mediated gene expression. The ARE Respone Element is also available as a pre-cloned transfection ready luciferase reporter vector on the LightSwitch™ Reporter System page under Synthetic Response Element Collection.
The TransAM® Method tab below provides complete method details; the kit manual can be downloaded under the Documents tab, while a selection of papers that cite the use of TransAM Nrf2 can be seen under the Publications tab.
Monitoring Nrf2 activation with the TransAM Nrf2 Kit.