FACE™ EGFR (Y1173) Chemi

by Active Motif
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48790

Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

Each FACE EGFR Kit provides 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total EGFR. The Y845, Y992 and Y1173 kit each contain phospho-EGFR antibody that recognizes EGFR only when phosphorylated at Tyr845, Tyr992 or Tyr1173, respectively. Each kit also contains a total-EGFR antibody that recognizes EGFR regardless of its phosphorylation state. Click the EGFR Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ EGFR (Y845) 1 x 96 rxns 48340 $525 Buy Now
FACE™ EGFR (Y845) 5 x 96 rxns 48840 $2,100 Buy Now
FACE™ EGFR (Y845) Chemi 1 x 96 rxns 48440 $580 Buy Now
FACE™ EGFR (Y845) Chemi 5 x 96 rxns 48940 $2,320 Buy Now
FACE™ EGFR (Y992) 1 x 96 rxns 48150 $525 Buy Now
FACE™ EGFR (Y992) 5 x 96 rxns 48650 $2,100 Buy Now
FACE™ EGFR (Y992) Chemi 1 x 96 rxns 48250 $580 Buy Now
FACE™ EGFR (Y992) Chemi 5 x 96 rxns 48750 $2,320 Buy Now
FACE™ EGFR (Y1173) 1 x 96 rxns 48190 $525 Buy Now
FACE™ EGFR (Y1173) 5 x 96 rxns 48690 $2,100 Buy Now
FACE™ EGFR (Y1173) Chemi 1 x 96 rxns 48290 $580 Buy Now
FACE™ EGFR (Y1173) Chemi 5 x 96 rxns 48790 $2,320 Buy Now
Measurement of phosphorylated EGFR (Y992) and total EGFR using the FACE EGFR Cell Based ELISA (In Cell Western) method.
 
Figure 1: Detection of EGFR (Y992) phosphorylation using FACE.

The FACE EGFR Kit was used to assay the level of phosphorylated EGFR (Y992) contained within EGF stimulated HeLa cells. FACE assays were performed before and 2, 10 and 30 minutes after EGF induction.

Antibody Specificities

The FACE phospho-EGFR (Tyr992), phospho-EGFR (Tyr1173) and phospho-EGFR (Tyr845) antibodies were raised in rabbits against synthetic phospho-peptides corresponding to residues surrounding phosphorylated Tyr992, Tyr1173 or Tyr845 of human EGF receptor. The phospho-EGFR (Tyr992), phospho-EGFR (Tyr1173) and phospho-EGFR (Tyr845) antibodies recognize EGFR only when phosphorylated at Tyr992, Tyr1173 and Tyr845 respectively. The total-EGFR antibody supplied in the FACE EGFR Kits recognizes EGFR protein regardless of its phosphorylation site.

EGFR Overview

Epidermal Growth Factor (EGF) Receptor (EGFR, HER-1, c-ErbB-1) is a member of the Epidermal Growth Factor Receptor family of Receptor Tyrosine Kinases. These cell surface receptors play an important role in the flow of information from the outside of a cell to the inside. Upon binding of EGF to the extracellular domain, the receptor undergoes dimerization and becomes phosphorylated on several tyrosine residues within the cytoplasmic domain. These result in EGFR activation and increased tyrosine kinase activity toward a variety of intracellular substrates. Autophosphorylation of tyrosine 845, 992 and 1173 are critical to EGFR sign

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