FACE™ JAK1 Chemi

by Active Motif
Available SKUs: 48785 |
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Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

FACE JAK1 Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total JAK1 (Janus Kinase 1). The phospho-JAK1 antibody recognizes JAK1 only when phosphorylated at Tyr1022 and Tyr1023. The total-JAK1 antibody recognizes JAK1 regardless of its phosphorylation state. Click the JAK1 Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ JAK1 1 x 96 rxns 48185 $525 Buy Now
FACE™ JAK1 5 x 96 rxns 48685 $2,100 Buy Now
FACE™ JAK1 Chemi 1 x 96 rxns 48285 $580 Buy Now
FACE™ JAK1 Chemi 5 x 96 rxns 48785 $2,320 Buy Now
Measurement of phosphorylated JAK1 (Y1022/Y1023) and total JAK1 using the FACE JAK1 Cell Based ELISA (In Cell Western) method.
 
Figure 1: Measurement of phosphorylated and total JAK1.

3T3-L1 cells were cultured in 96-well plates for 48 hours and converted to adipocytes. Cells were then serum-starved for 16 hours, treated with 20 ng/ml LIF for 10 minutes and fixed. Total and phospho JAK1 were each assayed in triplicate using the phospho and total JAK1 antibodies included in the FACE JAK1 Kit. Data was plotted after correction for cell number (performed through use of Crystal Violet).

Antibody Specificities

The phospho-JAK1 antibody is specific for phosphorylated JAK1 and was raised against a synthetic phospho-peptide corresponding to

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