FACE™ PI3 Kinase p85 Chemi

by Active Motif
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48775

Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

FACE PI3 Kinase p85 Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total PI3K p85. The phospho-PI3K p85 antibody preferentially recognizes peptides and proteins containing phospho-tyrosine at the consensus YXXM motif. The total-PI3K p85 antibody recognizes PI3K p85 regardless of its phosphorylation state. Click the PI3K p85 Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ PI3 Kinase p85 1 x 96 rxns 48175 $525 Buy Now
FACE™ PI3 Kinase p85 5 x 96 rxns 48675 $2,100 Buy Now
FACE™ PI3 Kinase p85 Chemi 1 x 96 rxns 48275 $580 Buy Now
FACE™ PI3 Kinase p85 Chemi 5 x 96 rxns 48775 $2,320 Buy Now
Measurement of phosphorylated PI3 Kinase p85 (YXXM) and total PI3 Kinase p85 using the FACE PI3 Kinase p85 Cell Based ELISA (In Cell Western) method.
 
Figure 1: Measurement of phosphorylated and total PI3 Kinase.

NIH/3T3 cells were cultured in 96-well plates and serum-starved for 16 hours. Cells were then treated with 50 ng/ml of PDGF for 5 minutes and fixed. Total and phospho PI3K p85 were each assayed in triplicate using the phospho and total PI3K p85 antibodies included in the FACE PI3 Kinase Kit. Data was plotted after correction for cell number (performed through use of Crystal Violet).

Antibody Specificities

The phospho-PI3K p85 antibody preferentially recognizes peptides and proteins containing phospho-tyrosine at the consensus YXXM motif. It was raised against a synthetic phospho-(Tyr) p85 binding motif peptide. Some cross-reactivity is observed for peptides that contain phospho-tyrosine followed by methionine. No cross-reactivity is observed with corresponding nonphosphorylated sequences or with other phospho-tyrosine-containing motifs. The total-PI3K p85 antibody recognizes the total level of PI3K p85 proteins regardless of the phosphorylation state.

PI3 Kinase p85 Overview

Phosphoinositide 3 Kinase (PI3 Kinase, PI3K) is a regulator of cell proliferation, survival and gr

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