FACE™ FKHR (FOXO1) Chemi

by Active Motif
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Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

FACE FKHR Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total FKHR (Forkhead). The phospho-FKHR antibody recognizes FKHR only when phosphorylated at Thr24, and also recognizes FKHRL1 phosphorylated at Thr32 and AFX phosphorylated at Thr28. The total-FKHR antibody recognizes FKHR regardless of its phosphorylation state. Click the FKHR Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ FKHR (FOXO1) 1 x 96 rxns 48160 $525 Buy Now
FACE™ FKHR (FOXO1) 5 x 96 rxns 48660 $2,100 Buy Now
FACE™ FKHR (FOXO1) Chemi 1 x 96 rxns 48260 $580 Buy Now
FACE™ FKHR (FOXO1) Chemi 5 x 96 rxns 48760 $2,320 Buy Now
Measurement of phosphorylated FKHR (T24) and total FKHR using the FACE FKHR Cell Based ELISA (In Cell Western) method.
 
Figure 1: Measurement of phosphorylated and total FKHR.

CHO-K1 cells were cultured in 96-well plates and serum-starved for 16 hours. Cells were then treated with 20% FBS for 30 minutes and fixed. Total and phospho FKHR were each assayed in triplicate using the phospho and total FKHR antibodies included in the FACE FKHR Kit. Data was plotted after correction for cell number (performed through use of Crystal Violet).

Antibody Specificities

The phospho-FKHR antibody is specific for phosphorylated FKHR and was raised against a synthetic phospho-peptide corresponding to residues surrounding Thr24 of human FKHR. This antibody recognizes FKHR only when phosphorylated at Thr24 and also recognizes FKHRL1 when phosphorylated at Thr32 and AFX when phosphorylated

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