FACE™ ErbB-2 (Y1248) Chemi

by Active Motif
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Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

Each FACE ErbB-2 Kit provides 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total ErbB-2. The Y877 kit contains phospho-ErbB-2 antibody that recognizes ErbB-2 only when phosphorylated at Tyr877, while the Y1248 kit provides a phospho-antibody that recognizes ErbB-2 only when phosphorylated at Tyr1248. Each kit contains a total-ErbB-2 antibody that recognizes ErbB-2 regardless of its phosphorylation state. Click the ErbB-2 Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ ErbB-2 (Y877) 1 x 96 rxns 48130 $525 Buy Now
FACE™ ErbB-2 (Y877) 5 x 96 rxns 48630 $2,100 Buy Now
FACE™ ErbB-2 (Y877) Chemi 1 x 96 rxns 48230 $580 Buy Now
FACE™ ErbB-2 (Y877) Chemi 5 x 96 rxns 48730 $2,320 Buy Now
FACE™ ErbB-2 (Y1248) 1 x 96 rxns 48105 $525 Buy Now
FACE™ ErbB-2 (Y1248) 5 x 96 rxns 48605 $2,100 Buy Now
FACE™ ErbB-2 (Y1248) Chemi 1 x 96 rxns 48205 $580 Buy Now
FACE™ ErbB-2 (Y1248) Chemi 5 x 96 rxns 48705 $2,320 Buy Now
Measurement of phosphorylated ErbB-2 (Y877), phosphorylated ErbB-2 (Y1248) and total ErbB-2 using the FACE ErbB-2 (Y877) and FACE ErbB-2 (Y1248) Cell Based ELISAs (In Cell Westerns).
 
Figure 1: Monitoring total- and phospho-ErbB-2 using FACE.

The FACE ErbB-2 (Y1248) and ErbB-2 (Y877) Kits were used to assay the levels of total and phosphorylated ErbB-2 contained within untreated or EGF treated A-431 cells.

Antibody Specificities

The FACE ErbB-2 (Y877) Kit contains a phospho-ErbB-2 antibody that was raised in rabbit against a synthetic phospho-peptide corresponding to residues surrounding phosphorylated Tyr877 of human ErbB-2 and recognizes ErbB-2 only when phosphorylated at Tyr877. The FACE ErbB-2 (Y1248) Kit contains a phospho-ErbB-2 antibody that was raised in rabbit against a synthetic phospho-peptide corresponding to residues surrounding phosphorylated Tyr1248 of human ErbB-2 and recognizes ErbB-2 only when phosphorylated at Tyr1248. The total-ErbB-2 antibody supplied in the FACE ErbB-2 Kits recognizes ErbB-2 protein regardless of its phosphorylation site.

ErbB-2 Overview

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have

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